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KMID : 0988920080060020128
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Intestinal Research
2008 Volume.6 No. 2 p.128 ~ p.134
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Cyclooxgenase-2 Expression in Chronic Inflammatory Bowel Disease: Comparing of Immunohistochemical Stain to Real-time PCR
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Park Young-Sook
Kim Nam-In
Lee Won-Mi
Kim Seong-Hwan
Lee Jun-Seok
Lim Chae-Young
Choi Jung-Ho
Son Byoung-Kwan
Kim Eun-Kyung
Jo Yun-Ju
Jeon Dae-Won
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Abstract
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Background/Aims: This study compared the results of cyclooxygenase-2 (COX-2) expression in inflammatory bowel disease and tuberculous colitis as evident by immunochemical staining and real time polymerase chain reaction (PCR).
Methods: Patients with ulcerative colitis (n=18), Crohn’s disease (n=7), tuberculous colitis (n=7) and 10 normal controls were included. Biopsied colonic mucosa was simultaneously used for immunohistochemical staining and real time PCR.
Results: Patients with inflammatory bowel disease and tuberculous colitis showed high COX-2 expression by both methods compared to the normal controls. In Crohn’s disease patients, the real time PCR value correlated well staining grade; this correlation was not evident in ulcerative colitis patients. In real time PCR, grossly normal colonic mucosa in ulcerative colitis also showed higher expression of COX-2 than normal mucosa.
Conclusions: Real time PCR value of COX-2 is more representative of inflammation state in inflammatory bowel disease than the value from immunohistochemical staining.
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KEYWORD
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Cyclooxygenase 2, Inflammatory Bowel Diseases, Immunohistochemistry, Polymerase Chain Reaction
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